KMID : 0359320170570030155
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Korean Journal of Veterinary Research 2017 Volume.57 No. 3 p.155 ~ p.158
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Evaluation of immunocontraceptive vaccine composed of gonadotropin-releasing hormone conjugated with granulocyte-macrophage colony-stimulating factor in male rats
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Park Byung-Joo
Kim Yong-Hyun Ahn Hee-Seop Han Sang-Hoon Go Hyeon-Jeong Lee Joong-Bok Park Seung-Yong Song Chang-Seon Lee Sang-Won Choi In-Soo
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Abstract
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Surgical castration performed to reduce male-associated problems results in pain and microbial infections in male animals. Therefore, immunocontraception, which is mediated by the animal¡¯s own antibodies against reproductive hormones, has been recommended as an alternative to surgical castration when considering the animal¡¯s welfare. In this study, a new immunocontraceptive vaccine composed of six tandem copies of gonadotropin-releasing hormone (GnRH) fused to rat granulocyte-macrophage colony-stimulating factor (GM-CSF) was developed, and its efficacy was evaluated in male rats. Three different doses (10, 50, and 100 ¥ìg) of recombinant GM-CSF?GnRH protein were injected three times at intervals of two weeks into male rats. The rats vaccinated with three doses of GM-CSF?GnRH produced a significantly higher level of antibodies against GnRH than that in the negative control rats. Severe atrophy of gonads was observed in rats vaccinated with three doses of GM-CSF?GnRH but not in the negative control rats. The results reveal that the new GnRH vaccine conjugated with rat GM-CSF induces efficient immunocontraception in male rats. This formulation of the immunocontraceptive vaccine would be applicable to both domestic and pet male animals.
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KEYWORD
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immunologic contraception, gonadotropin-releasing hormone, granulocyte-macrophage colony-stimulating factor, rats, vaccines
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